Reduced activity or imbalances in the gut microbiome have been associated with a variety of conditions, including obesity, type II diabetes, and many more. ViennaLab 16S Microbiome NGS Assay, combining library preparation and bioinformatic analysis, enables species-level classification of bacteria colonizing the human gut.

Intestinal Microbiome

• Composition and diversity of the human gut microbiome have been linked to a wide range of phenotypes in health and disease.
• Targeted sequencing of the variable regions in the 16S rRNA gene provides a rapid and easy way to assess the bacterial composition in the human intestine.
• The ViennaLab 16S Microbiome NGS Assay in conjunction with the ViennaLab Microbiome Analysis Webtool offers a powerful end-to-end solution combining library preparation and bioinformatic analysis. Diversity statistics, informative summary tables and graphical output are generated to facilitate the rapid review and comprehensive evaluation of the bacterial composition in the analyzed samples.
• The kits allow multiplexing of up to 288 samples and we recommend the Illumina MiSeq instrument (due to the minimum read length requirement of 2x250bp and sequencing output).

In order to address our customers’ needs and further facilitate the analysis of the human gut microbiome we have currently implemented several improvements such as:

• Fast and optimized 2-step protocol which minimizes set-up time.
• Easier set-up with all PCR1 primers now contained in a single tube.
• More balanced heterogeneity spacers to enhance library complexity for improved sequencing quality.
• Sequencing-ready libraries in less than 3 h.
• Elimination of PhiX spike-in results in lower reagent expenses, more samples per sequencing run and therefore lower costs per sample.
• Enhanced analysis pipeline with added QC and filtering steps allows more accurate species classification.

In addition, adhering to the environmentally friendly mindset of our ViennaLab products – we reduced the number of tubes in order to generate less waste.

For the assessment of the number of samples that can be sequenced with the used sequencing chemistry, the calculation of the sample pooling, and the assistance with the sample sheet generation, please refer to the ViennaLab MicrobeCalc™.

Are you interested to see how the final data generated with our Microbiome Analysis Webtool will be presented in order to facilitate the rapid review and comprehensive evaluation of the bacterial composition of your samples? Feel free to contact us via This email address is being protected from spambots. You need JavaScript enabled to view it. and inquire for a demo report.

Note 1: REF 9-131, 9-132, 9-133 differ only in Indexing Primer Sets. If you plan to sequence more than 96 samples on one flow cell, please order kits with different sets (e.g. Set A and Set B). Make sure that all individual libraries sequenced in the same pool have a unique indexing primer combination.
Note 2: Indexing primers of REF 9-131-16 overlap with REF 9-131 Set A.

For Research Use Only. Not for use in diagnostic procedure.