The ViennaLab NGS Assays apply PCR-based enrichment technique tailored to the needs of the diagnostic target.

The principle

ViennaLab Diagnostics provides the entire workflow from DNA to report generation.

Two-step PCR-based target enrichment for bacterial classification based on the 16S rRNA sequence:

  • In the first PCR, regions of interest are enriched by amplification.
  • The second PCR introduces specific dual-indices for subsequent sample assignment, as well as adaptors for the sequencing on Illumina platforms.
  • Species-level classification of bacterial composition with ViennaLab Microbiome Analysis Webtool.

 Workflow 16S Microbiome NGS Assay