Most oncogenic mutations are acquired in critical genes involved in growth-controlling processes or tumor suppression. Somatic genetic testing can reveal the presence of tumor actionable variants and therefore provide guidance for personalized medicine.
The ViennaLab Somatic Mutations NGS Assay enables unbiased analysis of somatic variants in 10 key cancer associated genes as well as gene fusion detection in 3 cancer driver genes.

Somatic Mutations NGS Assay

• Cancer is a leading cause of death worldwide. Somatic variants found in cancerous tumors might affect treatment decision and outcome by conferring resistance or susceptibility to specific therapies.¹
• Genetic analysis of tumors can guide clinical management of cancer patients by providing information on diagnosis, prognosis, as well as eligibility for targeted therapies.¹
• The ViennaLab Somatic Mutations NGS Assay offers a complete solution comprising library preparation and proprietary bioinformatic analysis software. Focused analysis of somatic variants includes SNVs and InDels in 10 key cancer associated genes as well as gene fusion detection in 3 cancer driver genes caused by structural variations. Some of the diseases covered by this panel are non-small cell lung cancer, colorectal cancer, melanoma, thyroid cancer, etc.
• Covered genes: ALK*, APC, BRAF, EGFR, ERBB2, KRAS, MET, NRAS, PIK3CA, RET*, ROS1*, SMAD4, TP53.
• Variants: SNVs, InDels, Fusions.*^#
• Assay type: Target enrichment by hybridization.
• Target region: Whole coding sequence of covered genes including exon-intron boundaries as well as hotspot introns for fusions* (Target size: 62 kb).
• Recommended sequencing instruments: Illumina MiSeq, MiniSeq, iSeq 100.
  _{^# Available on request}
  

Useful information: A template for calculation of final library molarity and sample sheet generation is provided in the NGS Assays support section.

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Note: REF 9-231 may contain different sets of Indexing primers. If you plan to sequence more than 16 samples on one flow cell, please mention this during ordering to your local ViennaLab distributor to guarantee unique indexing primer combinations.

For Research Use Only. Not for use in diagnostic procedure.