Most oncogenic mutations are acquired in critical genes involved in growth-controlling processes or tumor suppression. Somatic genetic testing can reveal the presence of tumor-associated variants and therefore provide relevant data in cancer research.

The ViennaLab Somatic Mutations NGS Assay enables analysis of somatic variants for research on 10 key cancer-associated genes as well as gene fusion detection in 3 cancer driver genes, for research purposes.

Somatic Mutations NGS Assay

• Cancer is a leading cause of death worldwide. Research on somatic variants found in cancerous tumors may contribute to a better understanding of mechanisms associated with resistance or susceptibility to specific therapies.¹
• The ViennaLab Somatic Mutations NGS Assay offers a complete solution comprising library preparation and proprietary bioinformatic analysis software. Focused analysis for research of somatic variants includes SNVs and InDels in 10 key cancer-associated genes as well as gene fusion detection in 3 cancer driver genes caused by structural variations. Some of the cancer types relevant to this research panel are non-small cell lung cancer, colorectal cancer, melanoma, thyroid cancer, etc.
• Covered genes: ALK*, APC, BRAF, EGFR, ERBB2, KRAS, MET, NRAS, PIK3CA, RET*, ROS1*, SMAD4, TP53.
• Variants: SNVs, InDels, Fusions.*^#
• Assay type: Target enrichment by hybridization.
• Target region: Whole coding sequence of covered genes including exon-intron boundaries as well as hotspot introns for fusions* (Target size: 62 kb).
• Recommended sequencing instruments: Illumina MiSeq, MiSeq i100 series, MiniSeq, iSeq 100.
  _{^# Available on request}
  

Useful information: A template for calculation of final library molarity and sample sheet generation is provided in the NGS Assays support section.

How can I analyze my sequencing data and summarize it in a meaningful report? Follow me.

Note: REF 9-231 may contain different sets of Indexing primers. If you plan to sequence more than 16 samples on one flow cell, please mention this during ordering to your local ViennaLab distributor to guarantee unique indexing primer combinations.

For Research Use Only. Not for use in diagnostic procedure.