The ViennaLab NGS Assay applies a PCR-based enrichment technique tailored to the needs of the diagnostic target.

The principle of amplicon based microbial NGS sequencing


The ViennaLab 16S Microbiome NGS Assays provide a complete workflow, from isolated DNA to report generation.

The procedure relies on a two-step PCR-based target enrichment for bacterial classification.

  • During the first PCR, regions of interest (e.g. 16S rRNA gene) are amplified.
  • The second PCR introduces specific dual-indices for subsequent sample assignment, as well as adaptors for the sequencing on Illumina platforms.
  • Species-level classification of bacterial composition with the ViennaLab Microbiome Analysis Webtool.

 

Workflow 16S Microbiome NGS Assay

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