The principle of amplicon based microbial NGS sequencing

The ViennaLab 16S Microbiome + ITS NGS Assays provide a complete workflow, from isolated DNA to report generation.

The procedure relies on a two-step PCR-based target enrichment for bacterial and fungal classification.

• During the first PCR, regions of interest (e.g. 16S rRNA gene and ITS2 region) are amplified.
• The second PCR introduces specific dual-indices for subsequent sample assignment, as well as adaptors for the sequencing on Illumina platforms.
• Species-level classification of bacterial and fungal composition with the ViennaLab Microbiome Analysis Webtool.